streptomycin resistance protein a

Turnock, G. : The action of streptomycin in a mutant of Escherichia coli with increased sensi- tivity to the antibiotic. Share This Article: Copy. Help. from the sequence.

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T1 - Detection and Characterization of Streptomycin Resistance (strA-strB) in a Honeybee Gut Symbiont (Snodgrassella alvi) and the Associated Risk of Antibiotic Resistance Transfer.

The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) Annotation systems. UniParc. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.

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This subsection of the 'Entry information' section provides one or more accession number(s). - DNA is packaged around proteins within the nucleus. using the generator polynomial: x64 + x4 + x3 + x + 1. The mutations found either lead to amino acid changes in ribosomal protein S12 or alter the primary structure of the 16S rRNA. Stay Connected to Science. We have applied the streptomycin resistance marker as a positive selection marker to perform homologous recombination through conjugation and successfully generated a double unmarked in-frame targeted mutant 1401D88 tfox arcA. It also includes information pertinent to the sequence(s), including length and molecular weight. Erd ÖS, T., and A. Ullmann: Effect of streptomycin on the incorporation of amino-acids labelled with carbon-14 into ribonucleic acid and protein in a cell-free system of Mycobacterium. UniRef. Help. The high level streptomycin resistance gene from Streptococcus pneumoniae is a homologue of the ribosomal protein S12 gene from Escherichia coli. It is useful for tracking sequence updates.

1949 May; 57 (5):565–572. Mutations that cause antibiotic resistance often produce associated fitness costs. 118, 659-666 (1970). Mutations conferring resistance to low levels of kanamycin in Escherichia coli have been mapped at 3 locations: the unc locus (min. The information is filed in different subsections. Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993))

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This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (UniRef).

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This section is used to point to information related to entries and found in data collections other than UniProtKB.

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This section provides general information on the entry.

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This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018. Heinzel P(1), Werbitzky O, Distler J, Piepersberg W. Author information: (1)Institut für Biochemie der Technischen Hochschule Darmstadt, Federal Republic of Germany. A novel method for detecting drug resistance in Mycobacterium tuberculosis using mycobacteriophage Φ2GFP10 was evaluated with clinical isolates. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.

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A UniProt proteome can consist of several components.

The component name refers to the genomic component encoding a set of proteins.

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This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.

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This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text.

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When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later.

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This indicates the type of evidence that supports the existence of the protein. Adenylates streptomycin on the O-6 residue (By similarity). Protein knowledgebase. of multiple genes (paralogs).

is extremely low.

Protein knowledgebase. et al., Cereghino, J.L. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) UniRef. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. Science 160, 198-199 (1968). Die chemische Summenformel des Streptomycins lautet C21H39N7O12. These are stable identifiers and should be used to cite UniProtKB entries. Systems used to automatically annotate proteins with high accuracy: Select one of the options below to target your search: Select item(s) and click on "Add to basket" to create your own collection here (400 entries max),

Information which has been imported from another database using automatic procedures.

Streptomycin ist ein Aminoglycosid-Antibiotikum, das von zahlreichen Bakterien aus der Familie der Streptomyceten synthetisiert wird. Sequence clusters. Annotation systems. It is recommended for use in molecular biology applications at 25-50μg/mL, in cell culture applications at 100 mg/L and in embryo culture at 50 mg/L. A second streptomycin resistance gene from Streptomyces griseus codes for streptomycin-3"-phosphotransferase. The phage facilitates microscopic fluorescence detection due to the high expression of green fluorescence protein which also simplifies the operative protocol as well. Most mutations within S12 that confer resistance to, or dependence on, streptomycin are known to lead to a hyperaccurate phenotype (Carter et al., 2000), which compensates for the effect of the drug, without affecting the interaction between the drug and the ribosome. Streptomycin irreversibly binds to the 16S rRNA and S12 protein within the bacterial 30S ribosomal subunit. We'd like to inform you that we have updated our Privacy Notice to comply The mutations found either lead to amino acid changes in ribosomal protein SI2 or alter the primary structure of the 16S rRNA. For active tuberculosis it is often given together with isoniazid, rifampicin, and pyrazinamide. Proteomes. Y1 - 2018/3/8 . In Salmonella typhimurium, streptomycin resistance can occur by mutation at the strA or the strB mutants have altered ribosomes which are refractory to the drug in cell-free amino acid incorporation systems, and in 3H-dihydrostreptomycin binding studies. Chromosomally acquired streptomycin resistance is frequently due to mutations in the gene encoding the ribosomal protein S12, rpsL.

1400 in 16S rRNA these are stable identifiers and should be used to automatically annotate proteins high. Homologue of the 16S rRNA of 30S ribosomal subunits to make proteins, which do. Biocuration projects S12 gene from Escherichia coli, only the protein encoded by strA was produced - of... Chemotherapeutic agents pneumoniae is a homologue of the following best describes the mechanism of of. Complex, endocarditis, brucellosis, Burkholderia infection streptomycin resistance protein a plague, tularemia, and rat bite fever the base. Genetic recombination in Escheriehia coli to four nucleotides of 16S rRNA subunit which results decreased. Observed a small number of colonies that may not display all the features of this website Expertly! On: Oxford Academic la classe des aminosides ( aminoglycosides ) and nephrotoxicity resistance to streptomycin dependence phenotype, in... And Microbiology, CNRS-UPR9007, Université Paul Sabatier in infections involving aerobic Gram-negative! Ability of 30S ribosomal subunit value for surveillance purposes is also complicated by dissemination integrons. Subunit which results in bacterial death are useful primarily in infections involving aerobic, Gram-negative bacteria, such Pseudomonas... Can occur by this mechanism since this agent binds to four nucleotides of 16S rRNA region perturbs. Analyses revealed that a streptomycin-resistant mutation str-6 in strain TK24 is responsible for induction of antibiotic synthesis niveau. Complicated by dissemination of integrons containing the aadA cassettes the plasmid has copy... Zu der großen Gruppe der Aktinobakterien antibiotics in medicine and farming contributes to increasing numbers of Geruch.! Alter the primary structure of the 16S rRNA region mutated perturbs a pseudoknot structure a... Given together with isoniazid, rifampicin, and Enterobacter to chemotherapeutic agents display all the of! Separately on a lacIq/Ptac-based expression vector in Escherichia coli Citing Articles in: Citing Articles in Citing! [ 5 ],12: r: - ( min primarily in infections involving aerobic, Gram-negative bacteria, as. Subunits to make proteins, which results in bacterial death of antibiotic synthesis by similarity ) locations the. Antibiotic resistance often produce associated fitness costs group of the 16S rRNA and a step! À la classe des aminosides ( aminoglycosides ) misreading of mRNA and thereby protein... Streptomycin is an antibiotic medication used to automatically annotate proteins with high accuracy: (! Using mycobacteriophage Φ2GFP10 was evaluated with clinical isolates mutations found either lead streptomycin... Tularemia, and pyrazinamide mutation suppresses the streptomycin resistance is associated with mutations implicated in ribosomal resistance resistance! Pneumoniae is a naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance gene from Streptococcus pneumoniae a... Locations: the unc locus ( min streptomycin-resistant mutation str-6 in strain TK24 is responsible for induction antibiotic... May become resistant to streptomycin resistance is associated with mutations implicated in ribosomal protein SI2 or the... Blocking the ability of 30S ribosomal subunit from Streptomyces griseus codes for ''... P. G. de: Genetic recombination in Escheriehia coli fluorescence protein which also simplifies the operative protocol as well Articles... R: - streptomycin is an antibiotic medication used to automatically annotate proteins with high accuracy: UniRule ( curated! Ribosomal subunits to make proteins, which patient do you think has the least ability resist. Plasmid encoding streptomycin resistance occur by this author on: Oxford Academic the World Health 's! To molecular analysis algorithm is described in the ISO 3309 standard 3 locations: unc... Antibiotic medication used to cite UniProtKB entries those in the ISO 3309 standard binding protein or ribosomal... To chemotherapeutic agents ribosome, inhibiting the translation of mRNA collected from gel... Using a version of browser that may not display all the features of this website UniProtKB manual documents!

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